Each kit includes:
• Vials of dried, pre-mixed substrates and internal standards (qNMR quantified) for MS/MS duplex enzyme assay of Palmitoyl Protein Thioesterase 1 and Tripeptidyl Peptidase 1 enzymes by mass spectrometry.
• The relevant Enzyme Assay Buffer.
First order comes with qNMR quantified enzymatic products relevant to the PPT1 and TPP1 enzyme assays, for mass spectrometry tuning and standard curve generation (See Absolute Quantification).
"Minimum order quantity: 5 vials (250 duplex assays)"
CLN1;CLN2;neuronal ceroid lipofuscinoses; NCL
Duplex Assay Cocktail of Palmitoyl Protein Thioesterase1 &Tripeptidyl Peptidase1
The mixture contains Phe-Ala-Ala-4-[6-(t-butyl formatamino)hexylaminoformyl]benzylamine (MW=638.80), t-butyl {6-[4-(aminomethyl)benzamido]hexyl}carbamate-d9 (MW=358.52), 4-methylumbelliferyl-6-thiomyristoyl-β-D-glucoside (MW=564.73), 4-methylumbelliferyl-6-thio-β-D-glucoside-13C4 (MW=358.35) and octyl glucoside detergent (MW =292.37).