Each kit includes:
• Vials of pre-mixed substrates and internal standards (qNMR quantified) dissolved in Assay Buffer for MS/MS duplex enzyme assay of Galactose-1-phosphate uridylyltransferase (GALT) and Biotinidase (BTD).
• The LgtC working solution (LgtC enzyme in the appropriate buffer).
First order comes with qNMR quantified enzymatic products relevant to the GALT and BTD enzyme assays, for mass spectrometry tuning and standard curve generation (See Absolute Quantification).
"Minimum order quantity: 10 vials (500 duplex assays)"
Duplex Assay Kit for Galactose-1-phosphate uridylyltransferase & Biotinidase
The mixture contains N-[3-(O-lactosyl)propyl]-12-[benzyl(phenyl)amino]dodecanamide (MW=762.9), 4-amido-biotin-N-(4-butylbenzyl)benzamide (MW=508.7), and 4-amino-N-(4-butylbenzyl)benzamide-D4 (MW=286.4).
Catalog Number: CD3