Each kit includes:
• Vials of dried, pre-mixed substrate and internal standard (qNMR quantified) for MS/MS enzyme assay of Lysosomal Acid Lipase (LIPA).
• A vial of ethanolic cardiolipin solution required as a LIPA activator.
• The relevant Enzyme Assay Buffer.
First order comes with qNMR quantified enzymatic product relevant to the LIPA enzyme assay, for mass spectrometry tuning and standard curve generation (See Absolute Quantification).
Note: Enfanos' LIPA substrate is 100% specific for LIPA; therefore, LIPA assays do not require duplicate assays done in parallel in the presence and absence of the LIPA inactivator Lalistat-2.
"Minimum order quantity: 5 vials (250 assays)"
Wolman disease; LAL; Lysosomal acid lipase
Lysosomal Acid Lipase Assay Cocktail
The mixture contains 8-Methyl-4-propyl-coumarin-7-yl palmitate (MW 456.7) and 7-Hydroxy-8-methyl-4-propyl-coumarin-13C4 (MW 218.2).
Catalog Number: CS2